1. Loaf of white bread was bought from the supermarket
2.The loaf was cut into 10 square pieces (3 x 3cm)
3.The 4 pieces of bread was labelled as experiment 1
4. The other 4 pieces of bread was labelled as experiment 2
5.Each bread was labelled 0%,5%,10%,20% in experiment 1
6.Repeat steps for experiment 2
7.Plastic container lids were prepared (8 lids)
8.The lids were divided in 2 groups
9.The first four lids were labelled as experiment 1
10. The other four lids were labelled as experiment 2
11. All the bread pieces were placed onto each lid according to its experiment number
12. All four empty sprayers were labelled ;0%,5%,10%,20%
13.From the measuring cup , 20ml of water was measured out and poured into sprayer bottle 0%
14.From the measuring cup, 1ml of bleach was poured into sprayer 5%
15.The measuring cup was rinsed and wiped clean
16.From the measuring cup 19ml of water was poured into sprayer 5%
17.From the measuring cup of 2ml of bleach was poured into sprayer 10%
18.The measuring cup was rinsed
19.A total of 18ml of water was measured from the measuring cup and poured into sprayer 10%
20.A total of 16ml of water was poured into sprayer bottle 20%
21.A total of 4ml of bleach was poured into sprayer bottle 20%
22.The bread pieces from experiment 1&2 with the 0% label was sprayed with the 0% sprayer
23.Repeat step 22 to the other bread pieces and their assigned sprayers
24.Spray assigned bread pieces with solutions three times a day for 7 days
25.Place all bread pieces on lids in a damp spot, preferably away from sunlight
26.Repeat step 15 three times a week
27.The bread pieces were observed everyday for 7 continuous days
28.The mould should not be moved or shaken during experiment
29.A table was drawn up to record any descriptions of mould growths or changes
30.Observations of the mould was recorded during the week everyday
31.Each assigned bread piece was thoroughly observed
32.Observations were recorded onto a table
33.The whole method should be repeated one more time to obtain more accurate results
(the experiment can be repeated using 4 bread pieces ,4 number of lids, half the number of chemicals. 3.5ml of chemical & 35ml of water
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